GENTAUR Contact Gentaur

GENTAUR Survey

GENTAUR EUROPE

+32 1658 9045
+32 1650 9045

BELGIUM1

tel +32 2 732 5688
fax +32 2 732 4414
info@gentaur.com
Av. de l' Armée 68
B-1040 Brussels

France

tel 01 43 25 01 50
fax 01 43 25 01 60
9, rue Lagrange
75005 Paris

Italy

tel 02 36 00 65 93
fax 02 36 00 65 94
20135 Milano

Germany

tel +32 16 58 90 45
fax +32 16 50 90 45
Forckenbeckstraße 6
D-52074 Aachen

Japan

tel +81 78 386 0860
fax +81 78 306 0296
Minaatojimaminami-manchi
Chuo-ku, Kobe
065-0047


MitoPTTM Detection of Mitochondrial Permeability

Detection of the mitochondrial permeability transition event (PT) provides an early indication of the initiation of cellular apoptosis. This process is typically defined as a collapse in the electrochemical gradient across the mitochondrial membrane, as measured by the change in the membrane potential (DY).

Loss of mitochondrial DY, indicative of apoptosis, can be detected by a unique fluorescent cationic dye, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzamidazolocarbo-
cyanin iodide, commonly known as JC-11. This dye has been incorporated into the user-friendly MitoPTT kit for the simple and reproducible detection of the PT event in apoptotic cells.

Figure 4: Negative cell control, Log FL1 (X-axis) and log FL2 (Y-axis)
R2 = 91.07% R3 = 8.08%.

Figure 5: Positive cell control, Log FL1 (X-axis) and log FL2 (Y-axis)
R2 = 60.93% R3 = 38.13%.

In Figures 4 and 5, cells were analyzed in a Becton Dickinson FACSCaliber Flow Cytometer. Jurkat cells were treated with either DMSO (negative, non-induced cells) or with staurosporine (apoptotic, induced cells) for 3 hours at 377C, then labeled with MitoPTT for 15 minutes. Collapse of the mitochondrial DY is indicated by an increase in the number of cells falling into R3 corresponding to a loss of red fluorescence, indicative of the onset of apoptosis. (In these figures of flow cytometer data, non-apoptotic cells are in R2, which prints in green, and apoptotic cells are in R3, which prints in pink.)

Reference:

1. Smiley, S. T., Reers, M., Mottola-Hartshorn, C., Lin, M., Chen, A., Smith, T. W., Steele, G.D., and Chen, L. B. Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate forming lipophilic cation JC-1. Proc. Natl. Acad. Sci. USA 88: 3671-3675 (1991).

 

send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Site powered by Acid Dragon (AC)
Last modified: 05/19/16