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FMD IgG Antibody ELISA test kit

Brief
The Swine Foot And Mouth Disease Antibody ELISA Test kit is used for the detection the FMD-O antibody (O type antibody of Swine Foot And Mouth Disease) in swine serum; assessing the immunity conditions against Swine Foot And Mouth Disease in the pig farm; and for the investigation of the epidemiology of the Swine Foot And Mouth Disease.
The test kit is made from the antigen coated microplate, enzyme conjugate (HRP goat-anti-pig IgG), and other reagents; it applies the indirect  ELISA  principle to test the antibody against O type antibody of Swine Foot And Mouth Disease in pig serum.

Principle
The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the Swine Foot And Mouth Disease. In the test, the diluted contrast serum and sample (to be tested) are added, and incubate; after that, if the sample exists FMD-O  specific antibody, it will be bound with the antigens of the microplate; eliminate the unbound antibody and other compositions by washing , and adding enzyme conjugate (anti-pig IgG-HRP) to specifically bind with the compound of antibody and antigen of the microplate. Then the unbound conjugate is removed by washing, add the TMB substrate in the well, to react with HRP into a blue product. After discontinuing the reaction by adding HF solution, test the OD value in each reaction well with ELIASA at 630nm wavelength.

Specifications
 

Reagents

1 Antigen coated microplate.................................................................2 pieces (96 wells)

2 Negative control serum(green lid).....................................................1 tube (1ml/tube)

Positive control serum (yellow lid).......................................................1 tube (1ml/tube)

3 Anti-pig IgG-HRP combination(yellow lid)..................................... 1 bottle (22ml/bottle)

4 20× concentrated washing liquid (white lid)....................................1 bottle (30ml/bottle)

5 Substrate A (red lid) ........................................................................... 1 bottle (12ml/bottle)

Substrate B (black lid).............................................................................1 bottle (12ml/bottle)

6 Stop solution (blue lid)........................................................................ 1 bottle (12ml/bottle)

7 Sample diluent (transparent lid).......................................................... 1 bottle (50ml/bottle)

 

 

Storage: store 2-8, not frozen.

Expiry date: 6 months

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Last modified: 05/19/16