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Survivin Header
Also called: Apoptosis Inhibitor 4 or API4                                                          Price: 655 Euro
human Total Survivin Colorimetric (EIA) Kit
Assay Format Catalog #
Kit Insert
MSDS
Assay Layout
total: 96 Well Kit 900-111
assay insert
Survivin assay
Product Features | Significance and Uses | Typical Standard Curves | Assay Procedure |
Performance Specs | Cross Reactivities | Related Products
Survivin ELISA assay
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.

Survivin ELISA features
Survivin poster presented at M.D. Anderson Cancer Center Tumor Markers Conference, 2003... learn more>>

Survivin Range: 31.25-1,000 pg/mL
Survivin Sensitivity: 3.6 pg/mL
Stable, Color-Coded Liquid Reagents


Survivin ELISA Uses

Survivin, a new inhibitor of apoptosis (IAP) protein, is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, Survivin associates with the microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of Survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It indicates that Survivin may counteract a default induction of apoptosis in G2/M phase. The over expression of Survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.


Survivin Curves
survivin ELISA curve

Survivin Procedures
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet the yellow Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet the blue Conjugate into the wells and incubtate.
6. Aspirate and wash the plate.
7. Pipet Substrate into the wells and incubate.
8. Pipet Stop solution and read plate on a plate reader at 450nm.
9. Calculate sample concentrations from the Standard Curve.

 


Survivin Performance

human Total Survivin (900-111)
RANGE
31.25-1,000 pg/mL
SAMPLE SIZE
100 µL
SAMPLES PER 96 WELL KIT
38 in duplicate
SENSITIVITY
3.6 pg/mL
PRECISION
1.3-2.4% Intra
5.9-17.5% Inter
SAMPLE TYPE
recommended dilution
Tissue Culture Media (none)
Cell Lysates ( 1:80)
Serum ( 1:2)
Plasma (Heparin) ( 1:4)
Urine ( 1:2)
SPECIES SPECIFICITY
human

Survivin ELISA Reactivities

Compound Cross Reactivity Compound Cross Reactivity
total Survivin 100% pJNK <0.1%
<0.1%: human MEK 1, pJNK, p300, Granzyme B, Caspase-3 or Caspase-9.
We are currently evaluating cross-reactivities of rat and mouse Survivin in this kit. Please contact us for suitability of applications to these sample types.

Survivin ELISA Related Products

Product Name Catalog Number
Caspase-3 Detection Kits   907-013  907-014
phospho ERK Assay   900-098
total ERK Assay   900-103
phospho p38 Assay   900-101
total p38 Assay   900-102

 

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Last modified: 05/19/16