GENTAUR Contact Gentaur

Up

GENTAUR Survey

GENTAUR EUROPE

+32 1658 9045
+32 1650 9045

BELGIUM1

tel +32 2 732 5688
fax +32 2 732 4414
info@gentaur.com
Av. de l' Armée 68
B-1040 Brussels

France

tel 01 43 25 01 50
fax 01 43 25 01 60
9, rue Lagrange
75005 Paris

Italy

tel 02 36 00 65 93
fax 02 36 00 65 94
20135 Milano

Germany

tel +32 16 58 90 45
fax +32 16 50 90 45
Forckenbeckstraße 6
D-52074 Aachen

Japan

tel +81 78 386 0860
fax +81 78 306 0296
Minaatojimaminami-manchi
Chuo-ku, Kobe
065-0047


SpinClean™ Glutathione Excellose® Kit
 
INTRODUCTION
 

The SpinClean™ Glutathione Excellose® kit provides fast and one step method for small-scale purification of GST fusion protein. It uses ready-to-use Glutathione Excellose® spin cloumn containing and centrifugation for rapid processing of multiple samples.

 

HOW TO USE
 


 

SpinClean™ Glutathione Excellose® kit contains Glutathione Excellose®,
spin column, collectionTube, and stock solution.

Protocols
1. Vortex the Glutathione Excellose tube and pipet 0.5ml of the slurry to Spin Column, add 0.5 ml of Equilibration Buffer in the Spin Column, and centrifuge at 700 x g (approx. 2,000 rpm) for 2 min.
2. Discard the eluate, load up to 0.5 ml of cell lysate, centrifuge,
and collect the flow-through fraction.
3. Add 0.5 ml of Washing Buffer, centrifuge, and collect the wash fraction.
4. Repeat step 3, if necessary.
5. Add 0.2 ml of Elution Buffer and centrifuge.
6. Collect the eluate


 



 

ORDERING INFORMATION
 


 

Catalog No
Package
21020
20 columns/pkg
21050
50 columns/pkg

 

FIGURE
 


 

GST fusion protein purification
E.coli lysate containing GST fusion protein was loaded to Glutathione Excellose® spin column.

1 : E. coli lysate
2 : flow-through fraction
3 : wash fraction
4 : purified GST
 


 

Home Up

send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Site powered by Acid Dragon (AC)
Last modified: 05/19/16