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fax 01 43 25 01 60
9, rue Lagrange
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Italy

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fax 02 36 00 65 94
20135 Milano

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065-0047


Sera and Media

 

 

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SERA

 

Fetal Bovine Serum (FBS)

BIOCHROM AG, Germany offers FBS, originated from four different sources, Australia , USA , EU- and USDA – approved countries. FBS offered by BIOCHROM AG are tested for Endotoxin, Mycoplasma, Bacterial L-forms, Viral contamination, Viral antibodies against BVD-MD, BHV-1, PI-3.

BIOCHROM AG also offers pre-tested FBS lots suitable for Amniotic Fluid cells, Hybridoma cells and Embryonic Stem cells.

Ordering Information

Cat.No

Product

Units

S 0113

Fetal Bovine Serum (FBS) (origin: EU-approved countries)

100ml

S 0115

Fetal Bovine Serum (FBS) (origin: EU-approved countries)

500ml

S 0213

Fetal Bovine Serum (FBS) (origin: USDA-countries)

100ml

S 0215

Fetal Bovine Serum (FBS) (origin: USDA-countries)

500ml

S 0313

Fetal Bovine Serum (FBS) (origin: USA)

100ml

S 0315

Fetal Bovine Serum (FBS)(origin: USA)

500ml

S 0413

Fetal Bovine Serum (FBS) (origin: Australia)

100ml

S 0415

Fetal Bovine Serum (FBS) (origin: Australia)

500ml

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Cell Culture Media

 

BIOCHROM liquid and powdered cell culture media are standardized according to the original formulation as recommended by the 'Tissue CultureAssociation'.Any modifications from the original formulation are indicated on the label.

Defined cell culture media generally consist of four basic chemical groups: amino acids, carbohydrates, anorganic salts and vitamins. The following is the list of Cell culture media offered by BIOCHROM.

Ordering Information

Cat.No

Product

Units

F 0915

Alpha Medium with 2.0g/l NaHCO3 with nucleosides

500ml

F 0925

Alpha Medium with 2.0g/l NaHCO3 without nucleosides

500ml

F 0225

BME with Earle's Salts with 2.2g/l NaHCO3

500ml

F 0235

BME with Earle's Salts (10X) without NaHCO3

500ml

F 0245

BME with Hanks' Salts with 0.35g/l NaHCO3

500ml

F 0565

CMRL 1066 with 2.2g/l NaHCO3

500ml

F 0855

Coon's F-12 mod. with 2.5g/l NaHCO3, without L-Glutamine

500ml

F 0405

DMEM with 3.7g/l NaHCO3, without Glucose

500ml

F 0413

DMEM with 3.7g/l NaHCO3, with 1.0g/l Glucose

100ml

F 0415

DMEM with 3.7g/l NaHCO3, with 1.0g/l Glucose

500ml

FG 0415

DMEM with 3.7 g/l NaHCO3, with 1.0g/l Glucose, with stable glutamine

500ml

F 0425

DMEM B723w.3.7g/l NaHCO3, w.4.5g/l Gluc, w/oL-Glut.w/oNa-Pyr.w.20mM HEPES 3

500ml

F 0435`

DMEM with 3.7g/l NaHCO3,
with 4.5g/l Glucose w/oNa-Pyruvate.

500ml

FG 0435

DMEM with 3.7g/l NaHCO3, with 4.5g/l Glucose, w.stable glutamine w/o Na-Pyrv

500ml

F 0445

DMEM with 3.7 g/l NaHCO3,
with 4.5 g/l Glucose with Na-Pyruvate.

500ml

FG 0445

DMEM with 3.7g/l NaHCO3, with 4.5g/l Glucose w.stable glutamine w.Na-Pyrv

500ml

F 0455

DMEM (10X) with 4.5g/l Glucose, without NaHCO3, w/oL-Glut., w/o Na-Pyrv

500ml

F 1445

VLE-DMEM (low endotoxn) w 4.5g/l gluc,w
3.7g/l NaHCO3, wNa-Pyr

500ml

FG 1445

VLE-DMEM w 4.5g/l gluc, w 3.7g/l NaHCO3, w Na-Pyr,w stab glut

500ml

F 0475

DMEM with 4.5g/l Glucose, with Na-Pyruvate, w/o Phenol red

500ml

T 041-01

DMEM Powder Medium with 1.0g/l Glucose

1litre

T 041-05

DMEM Powder Medium with 1.0g/l Glucose

5litres

T 041-10

DMEM Powder Medium with 1.0g/l Glucose

10litres

T 041-50

DMEM Powder Medium with 1.0g/l Glucose

50litres

T 043-01

DMEM Powder Medium with 4.5g/l Glucose

1litre

T 043-05

DMEM Powder Medium with 4.5g/l Glucose

5litres

T 043-10

DMEM Powder Medium with 4.5g/l Glucose

10litres

T 043-50

DMEM Powder Medium with 4.5g/l Glucose

50litres

F 4815

DMEM/Ham's F-12 (1:1) with 4.5g/l Glucose

500ml

FG 4815

DMEM/Ham's F-12 (1:1) with 4.5g/l Glucose with stable glutamine

500ml

T 481-01

DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose

1litre

T 481-05

DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose

5litres

T 481-10

DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose

10litres

T 481-50

DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose

50litres

F 0525

Glasgow-MEM with 2.A15675 g/l NaHCO3

500ml

F 0555

Grace's Insect cell medium

500ml

F 0713

Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO3

100ml

F 0715

Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO3

500ml

FG 0713

Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO3, with stable glutamine

100ml

FG 0715

Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO3, with stable glutamine

500ml

F 0723

Ham's F-10 without phenol red, with 1.2g/l NaHCO3, with 10mM HEPES

100ml

T 071-01

Ham's F-10 Powder Medium

1litre

T 071-05

Ham's F-10 Powder Medium

5litres

T 071-10

Ham's F-10 Powder Medium

10litres

T 071-50

Ham's F-10 Powder Medium

50litres

F 0815

Ham's F-12 with 10 mg/l phenol red, with 1.176g/l NaHCO3

500ml

FG 0815

Ham's F-12 with 10 mg/l phenol red, w. 1.176g/l NaHCO3, with stable glutamine

500ml

F 0465

Iscove's Medium, not compl., w.15mg/l phenol red, w. 3.024g/l NaHCO3

500ml

FG 0465

Iscove's Medium, not compl.,w.15mg/l phenol red, w.3.024g/l NaHCO3, w.Glut.B827

500ml

F 1315

L-15 Leibovitz Medium

500ml

F 1013

Mc-COY's 5 A mod. Medium with 2.2 g/l NaHCO3

100ml

F 1015

Mc-COY's 5 A mod. Medium with 2.2 g/l NaHCO3

500ml

F 0613

Medium 199 with Earle's Salts, with 2.2 g/l NaHCO3

100ml

F 0615

Medium 199 with Earle's Salts, with 2.2 g/l NaHCO3

500ml

FG 0615

Medium 199 with Earle's Salts, with 2.2 g/l NaHCO3, with stable glutamine

500ml

F 0633

Medium 199 with Hanks' Salts, with 0.35 g/l NaHCO3

100ml

F 0635

Medium 199 with Hanks' Salts, with 0.35 g/l NaHCO3

500ml

F 0663

Medium 199 with Earle's Salts, with 20mM HEPES without NaHCO3

100ml

F 0665

Medium 199 with Earle's Salts, with 20mM HEPES without NaHCO3

500ml

T 061-01

Medium 199 - Powder Medium with Earle's Salts

1litre

T 061-05

Medium 199 - Powder Medium with Earle's Salts

5litres

T 061-10

Medium 199 - Powder Medium with Earle's Salts

10litres

T 061-50

Medium 199 - Powder Medium with Earle's Salts

50litres

F 0315

MEM with Earle's Salts with 0.85 g/l NaHCO3

500ml

F 0325

MEM with Earle's Salts with 2.2 g/l NaHCO3

500ml

FG 0325

MEM with Earle's Salts with 2.2 g/l NaHCO3 with stable glutamine

500ml

F 0333

MEM with Earle's Salts (10X) without NaHCO3

100ml

F 0335

MEM with Earle's Salts (10X) without NaHCO3

500ml

F 0345

MEM with Hanks' Salts with 0.35 g/l NaHCO3

500ml

F 0383

MEM with Earle's Salts w/o NaHCO3, w/o phenol red

100ml

F 0385

MEM with Earle's Salts w/o NaHCO3, w/o phenol red

500ml

F 4315

MEM with Earle's Salts with 20mM HEPES without NaHCO3

500ml

T 437-01
 

MEM-Powder Medium with Earle's Salts
with non-essential amino acids

1litre

T 437-05
 

MEM-Powder Medium with Earle's Salts
with non-essential amino acids

5litres

T 437-10
 

MEM-Powder Medium with Earle's Salts
with non-essential amino acids

10litres

T 437-50

MEM-Powder Medium with Earle's Salts with non-essential amino acids

50litres

F 1213

RPMI 1640 with 2.0g/l NaHCO3, without L-Glutamine

100ml

F 1215

RPMI 1640 w. 2.0g/l NaHCO3, w/o L-Glutamine

500ml

FG 1213

RPMI 1640 with 2.0g/l NaHCO3, with stable glutamine

100ml

FG 1215

RPMI 1640 with 2.0g/l NaHCO3, w stable glut. (also available as “low endotoxin”)

500ml

F 1223

RPMI 1640 (10x) without NaHCO3

100ml

F 1225

RPMI 1640 (10x) without NaHCO3

500ml

F 1233

RPMI 1640 with 20mM HEPES, without NaHCO3

100ml

F 1235

RPMI 1640 with 20mM HEPES, without NaHCO3

500ml

FG 1233

RPMI 1640 without NaHCO3, with 20mM HEPES, with stable glutamine

100ml

FG 1235

RPMI 1640 without NaHCO3, with 20mM HEPES, with stable glutamine

500ml

F 1245

RPMI 1640 with 2.0g/l NaHCO3, without Methionine

500ml

F 1255

RPMI 1640 with 2.0g/l NaHCO3, with 40mg/l folic acid

500ml

FG 1255

RPMI 1640 with 2.0g/l NaHCO3, with 40mg/l folic acid, with stable glutamine

500ml

F 1275

RPMI 1640 with 2.0g/l NaHCO3 without phenol red

500ml

FG 1383

RPMI 1640 w 2.0g/l NaHCO3, 25mM HEPES, 5.5g/l NaCl, 5mg/l phenol red, stable glut.

100ml

FG 1385

RPMI 1640 w 2.0g/l NaHCO3, 25mM HEPES, 5.5g/l NaCl, 5mg/l phenol red, stable glut.

500ml

F 1295

RPMI 1640 with 2.0g/l NaHCO3, with 5mg/l phenol red

500ml

F 1415

VLE-RPMI 1640 (very low endotoxin), Endotoxin<0,01 IU/ml

500ml

FG 1415

VLE-RPMI 1640 (very low endotoxin) Endotoxin<0,01 IU/ml with stable glut.

500ml

T 121-01

RPMI 1640 - Powder Medium

1litre

T 121-05

RPMI 1640 - Powder Medium

5litres

T 121-10

RPMI 1640 - Powder Medium

10litres

T 121-50

RPMI 1640 - Powder Medium

50litres

F 0545

Insect cell medium TC 100

500ml

F 1115

Williams' Medium E with 2.2g/l NaHCO3, without L-Glutamine

500ml

F 1125

Williams' Medium E with 2.2g/l NaHCO3, without L-Glutamine, without phenol red

500ml

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Complete Culture Media

 

The Complete cell culture media are serum and protein free cell culture media which support the growth of different cells / cell lines. There are specific complete culture media are available to support the growth of cells / cell lines like, human amniotic fluid cells, FRTL 5, human and porcine hepatocytes, hybridomas, lymphocytes, human keratinocytes, CHO cells, VERO cells, sebocytes, neuronal cells, neuroblastoma x glioma hybrid cells, cytotoxicT-cells, chondrocytes, human endothelial cells etc.

Ordering Information

Cat.No

Product

Units

F 5043

Amniomed

100ml

F 5143

Amniomed with 25 mM HEPES

100ml

F 5013

Chromosome medium A without Phytohemagglutinin (PHA) L

100ml

F 5023

Chromosome A110medium B with Phytohemagglutinin (PHA) E

100ml

F 2235

Chondromed complete

500ml

F 2245

endomed complete

500ml

L 6211

Colchicin, 10 ug/ml in PBS, without Ca, Mg

25ml

L 6221

Colcemide, 10 ug/ml in PBS without Ca, Mg, in plastic bottles

25ml

L 6231

Colcemide, 10 µg/ml in HBSS

25ml.

F 8085
 

octomed serum free CHO-Medium with 2.2 g/l NaHCO3, without L-Glutamine

500ml

F 2225

fibromed complete

500ml

F 2255

heparmed Liver cell Medium

500ml

F 5515

Hybridoma Medium without HAT, with L-Glutamine, with 10% FBS

500ml

F 8035

HybridoMed DIF1000 serum free medium, for 10%CO2 conc., in plastic bottles

500ml

F 8055

HybridoMed DIF1000 serum free+A201medium, for 5%CO2 conc.,in plastic bottl.

500ml

F 8105

MCDB 153 Basal Medium without L-Glutamine

500ml

F 8115

MCDB 153 Basal Medium with L-Glutamine,
with 1.176 g/l NaHCO3

500ml

F 2265

Osteomed complete

500ml

F 8045

PFEK-1, protein free Medium for VERO-cells

500ml

F 8205

sebomed Basal Medium

500ml

F 8215

sebomed Complete Medium

500ml

F 8075

Start V Medium

500ml

F 8023

TNB 100 Medium in plastic bottles

100ml

F 8820

TNB 100, Lipid-Protein-Complex (necessary for 100 ml TNB 100)

2.5ml

F 0483

HAT-Supplement

100ml

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Cell Attachment Factors

 

Growth and differentiation of anchorage dependent cells are strongly influenced by the glass or plastic culture flasks offered as substrate. Cell growth rates can often be improved by surface coating with attachment factors such as fibronectin, collagen, gelatine or polylysine. With a collagen coating, survival time of hepatocytes cab be extended from normally one week to four weeks.

.

Ordering Information

Cat.No

Product

Units

L 7117

Fibronectin from human plasma

1mg

L 7127

Fibronectin from bovine plasma

1mg

L 7220

Collagen A, 1mg/ml

1 x (6 x 5ml)

L 7213

Collagen G

100ml

L 7230

Gelatine 10mg/ml

1 x (6 x 5ml)

L 7240

Polylysin MG>30.000; 0.1mg/ml

1 x (6 x 5ml)

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Tissue Disintegration Enzymes

 

Cells of animal tissue are attached to each other by a complex matrix of proteins, lipids, glycolipids and muco-polysaccharides. To isolate individual cells or to stat primary cultures, the delicate matrix must be partially broken down without destroying the cell or its surface.

Collagenase are the enzymes of choice for this application, if dissolved in balanced salts.

Ordering Information

Cat.No

Product

Units

C 1-28

Collagenase type I, CLS

100mg

C 1-22

Collagenase type I, CLS

1g

C 2-28

Collagenase type II, CLS II

100mg

C 2-22

Collagenase type II, CLS II

1g

C 3-28

Collagenase type III, CLS III

100mg

C 3-22

Collagenase type III, CLS III

1g

C 4-28

Collagenase type IV, CLS IV

100mg

C 4-22

Collagenase type IV, CLS IV

1g

L 2113

EDTA (Versen) 1% in PBS without Ca2+, Mg2+

100ml  

L 2123

Trypsin (1:250) 0.25% in PBS without Ca2+, Mg2+

100ml

L 2133

Trypsin (1:250) 2.5% in PBS without Ca2+, Mg2+

100ml

L 2153

Trypsin/EDTA Solution(10X)0.5%/0.2% in (10x) PBS,w/o Ca2+, Mg2+, in pl.B911 bott.

100ml

L 2163

Trypsin/EDTA solution 0.25%/0.02% in PBS without Ca2+, Mg2+

100ml

L 2103

Trypsin powder substance

100g

L 2223

Papain Solution (6.25mg/l)

100ml

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Seperating Solutions & Lectins

 

Cells of animal tissue are attached to each other by a complex matrix of proteins, lipids, glycolipids and muco-polysaccharides. To isolate individual cells or to stat primary cultures, the delicate matrix must be partially broken down without destroying the cell
or its surface.

Collagenase are the enzymes of choice for this application, if dissolved in balanced salts.

Ordering Information

Cat.No

Product

Units

L 6113

Biocoll-Separating Solution, density 1.077g/ml isoton

100ml

L 6115

Biocoll-Separating Solution, density 1.077g/ml isoton

500ml

L 6125

Biocoll-Separating Solution, density 1.09g/ml isoton

500ml

L 6155

Biocoll-Separating Solution, density 1.10g/ml isoton

500ml

L 6133

Percoll Separating Solution, density 1.077g/ml isoton

100ml

L 6135

Percoll Separating Solution, density 1.077g/ml isoton

500ml

L 6143

Percoll Separating Solution, density 1.124g/ml isoton

100ml

L 6145

Percoll Separating Solution, density 1.124g/ml isoton

500ml

L 6163

Percoll Separating Solution 40 %, density 1.055g/ml at +20°C

100ml

L 6173

Percoll Separating Solution 80 %, density 1.02g/ml at +20°C

100ml

M 5030

Phytohemagglutinine PHA L

1x(6x5ml)

M 5040

Phytohemagglutinine PHA E

1x(6x5ml)

M 5050

Concanavalin A (Con A)

1x(6x5ml)

M 5060

Pokeweed mitogen (PWM)

1x(6x5ml)

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Antibiotics

 

Cell culture media provide an ideal environment also for rods such as bacteria and fungi. Basically, cell cultures can be protected against contamination by strict adherence to sterile technique; but only the discovery of antibiotics made advanced cell culture

possible. However, as antibiotics have a toxic potental, their dosage must closely be monitored.

Ordering Information

Cat.No

Product

Units

A 2610

Amphotericin B, 250 µg/ml, lyophilized

1 x (6 x 5ml)

A 2612

Amphotericin B, 250 µg/ml

50ml

A 2812

Partricin

50ml

A 2710

Gentamycin 10 mg/ml, lyophilized

1 x (6 x 5ml)

A 2712

Gentamycin 10 mg/ml

50ml

A 271-23

Gentamycin-Sulfate

2.5g

A 271-25

Gentamycin-Sulfate

10g

A 271-26

Gentamycin-Sulfate

25g

A 2512

Kanamycin 5 mg/ml

50ml

A 231-40

Polymyxin B-Sulfate, approx. 6.000 - 8.000 U/mg

5 Mio IE

A 2412

Neomycin/Bacitracin 50 U/ml

50ml

A 2210

Penicillin/Streptomycin 10.000 U/10.000 µg/ml, lyophilized

1 x (6 x 5ml)

A 2212

Penicillin/Streptomycin 10.000 U/10.000 µg/ml

50ml

A 2213

Penicillin/Streptomycin 10.000 U/10.000 µg/ml

100ml

A 321-42

Penicillin

25 Mio IE

A 321-44

Penicillin

100 Mio IE

A 331-26

Streptomycin –Sulfate

25g

A 331-27

Streptomycin –Sulfate

100g

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Balanced Salts & Buffers

 

 

Ordering Information

Cat.No

Product

Units

L 6015

Alsever Solution pH 6.1

500ml

L 1913

Earle's Saline Solution (EBSS) with 2.2g/l NaHCO3

100ml

L 1915

Earle's Saline Solution (EBSS) with 2.2g/l NaHCO3

500ml

L 1925

Earle's Saline Solution (EBSS) (10X) without NaHCO3

500ml

L 2013

Hanks' Saline Solution (HBSS) with 0.35g/l NaHCO3

100ml

L 2015

Hanks' Saline Solution (HBSS) with 0.35g/l NaHCO3

500ml

L 2025

Hanks' Saline Solution (HBSS) (10X) without NaHCO3

500ml

L 2035

Hanks' Saline Solution (HBSS) without phenol red, with 0.35g/l NaHCO3

500ml

L 2045

Hanks' Saline Solution (HBSS) w/o Ca2+, Mg2+,w/o phenol red,w.0.35g/l NaHCO3

500ml

L 2053

Hanks' Saline Solution (HBSS) w/o Ca2+, Mg2+,w. phenol red, w.0.35g/l NaHCO3

100ml

L 2055

Hanks' Saline Solution (HBSS) w/o Ca2+, Mg2+, w. phenol red,w.0.35g/l NaHCO3

500ml

L 201-01

Hanks' Salts powder substance without NaHCO3

1litre

L 201-05

Hanks' Salts powder substance without NaHCO3

5litres

L 201-10

Hanks' Salts powder substance without NaHCO3

10litres

L 201-50

Hanks' Salts powder substance without NaHCO3

50litres

L 1613

HEPES buffer solution (1M) (50X)

100ml

L 1603

HEPES powder substance

100g

L 1713

Sodium bicarbonate (7.5%) NaHCO3

100ml

L 1703

Sodium bicarbonate NaHCO3 powder substance

100g

L 1815

PBS Solution with Ca2+, Mg2+

500ml

L 1823

PBS Solution without Ca2+, Mg2+

100ml

L 1825

PBS Solution without Ca2+, Mg2+

500ml

L 1835

PBS Solution (10X) without Ca2+, Mg2+

500ml

L 182-01

PBS-powder substance without Ca2+, Mg2+

1litre

L 182-05

PBS-powder substance without Ca2+, Mg2+

5litres

L 182-10

PBS-powder substance without Ca2+, Mg2+

10litres

L 182-50

PBS-powder substance without Ca2+, Mg2+

50litres

L 0015

Ultra Pure Water in glass bottle, sterile

500ml

L 0020

Ultra Pure Water, sterile, in Duran bottles suitable for HPLC

1litre

L 0030

Ultra Pure Water, not sterile

5litres

L 0040

Ultra Pure Water, not sterile

10litres

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Cell Culture Reagents

 

Ordering Information

Cat.No

Product

Units

L 6510

Heparin stabilizer free 5000U/ml lyophilized (for 5ml)

1 x (6 x 5ml)

L 0473

Sodium pyruvate 100mM

100ml

K 0293

Non-essential amino acids (NEA) (100X)

100ml

K 0363

MEM Amino Acids (50X)

100ml

K 0373

MEM Vitamins (100X)

100ml

K 0280

L-Glutamine (200mM)

1 x (24 x 2.5ml)

K 0281

L-Glutamine (200mM), lyophilized

1 x (6 x 5ml)

K 0282

L-Glutamine (200mM) in plastic bottles

50ml

K 0202

N-Acetyl-L-alanyl-L-glutamine, 200mM (stable glutamine)

50ml

L 3010

Biotect Protection Medium

24 x 2.5ml

L 6413

KCI solution (hypotonic) 0.075M

100ml

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Cell Culture Diagnostics

 

Ordering Information

Cat.No

Product

Units

F 0483

HAT-Supplement (50X)

100ml

F 0493

HT-Supplement (50X)

100ml

D 7001

Mycoplasma test - DNA-staining kit

1Kit

L 2203

Phenol red 0.5 %

100ml

L 6313

Neutral red (1:300 - 0.3%)

100ml

L 6323

Trypan blue 05+A2% in phys. Saline Solution

100ml

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