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Recombinant Mouse Interleukin 4 (rmIL-4)

 

 

Description

 

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant mouse IL-4 is a 13.5kDa globular protein containing 120 amino acid residues.

 

 

Specifications and Use

 

Source

 

l          E.coli

Molecular Mass

 

    13.5kDa.

 

Purity

 

l          > 95%, as determined by SDS-PAGE method.

Biological Activity

l          The ED50 was determined by the dose-dependent proliferation of mouse HT-2 cells is < 2.0ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.

   

Endotoxin Level

 

l          Less than 1EU/mg of rmIL-4 as determined by LAL method.

Formulation

 

l         Sterile filtered lyophilized (freeze-dried) powder

 

Solubility

l         It is recommended to reconstitute the lyophilized rmIL-4 in sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 5mg/ml of the cytokine.

 

Stability

 

l          Lyophilized protein is stable for a few weeks at room temperature, but best stored at -20.

l          Reconstituted mouse IL-4 should be stored in working aliquots at -20.

l          Avoid repeated freeze-thaw cycles.

 

Usage

l          FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.

 

 

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Last modified: 05/19/16