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Recombinant Mouse Flt3 Ligand (rmFlt3-L)

 

 

 

Description 

 

Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand has no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform.

 

 

Specifications and Use

 

Source

 

l          Escherichia coli.

 

Molecular Mass

 

l          16.4kDa

Purity

 

l          ³ 95% as determined by SDS-PAGE and HPLC analyses.

 

Biological Activity

l          rmFlt3-L is fully biologically active when compared to standards. Its specific activity is ³1×105 units/mg.

 

Endotoxin Level

 

l          Less than 1EU/µg of rmFlt3-L as determined by LAL method.

 

Formulation

 

l          Sterile filtered lyophilized (freeze-dried) powder, with 50mM Tris,100mM NaClpH8.5

 

Solubility

 

 

l          It is recommended to reconstitute the lyophilized rmFlt3-L in sterile distilled H2O to prepare a stock solution of no less than 100 mg/ml of the cytokine.  

 

Stability

l          The lyophilized rmFlt3-L powder is stable for a few weeks at room temperature, but best stored at -20.

l          Reconstituted rmFlt3-L aliquots should be stored at –20oC for maximal stability up to six months.

l          Avoid repeated freeze-thaw cycles.

 

Usage

l          FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.

 

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Last modified: 05/19/16