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Recombinant Human Interleukin 17  (rHuIL-17)

 

Description

 

The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 132 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and mouse cells. Recombinant human IL-17A is a 31.0kDa disulfide-linked homodimer of two 136 amino acid polypeptide chains.

 

 

Specifications and Use

 

Source

 

l          Escherichia coli.

Molecular Mass

 

l          15kDa

 

Purity

 

l          Greater than 95% by SDS-PAGE analyses.

Biological Activity

l          The ED50 as determined by the dose-dependent induction of IL-6 in Hs68 cell line was found to be approximately 2ng/ml.

 

Endotoxin Level

 

l          Less than 1EU/µg.

Formulation

 

l       Sterile filtered lyophilized (freeze-dried) powder

 

Solubility

l        It is recommended to reconstitute the lyophilized rHuIL-17 in sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 1mg/ml of the cytokine.

 

Stability

 

l          The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20.

l          Reconstituted human IL-17 should be stored in working aliquots at -20.

l          Avoid repeated freeze-thaw cycles.

 

Usage

l          FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.

 

 

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Last modified: 05/19/16