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Recombinant Human Interferon g (rHuIFN-g)

 

 

Specifications and Use

 

Description

l          Recombinant human IFN-γ produced in E. coli. is a non-covalent Dimer non-glycosylated, containing 143 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 16.5kD.

 

Source

 

l          E. coli.

Molecular Mass

 

l         Approximately 16.5kD.

 

Purity

 

l          97%, as determined by SDS-PAGE and HPLC method.

Endotoxin Level

 

l          1EU/mg, determined by the LAL method.

Biological Activity

l          Bioactivity is detected using WISH cell (a heteroploid human amnion cell line) as dependent cell strain. The specific activity shall be not less than 2×107 IU/mg of protein.
 

Formulation

 

l          Lyophilized from a 0.2mm filtered solution in 20mM Phosphate buffer.

 

Reconstitution

l          It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 10 mg/ml of the cytokine.

 

Storage

 

l          Lyophilized samples are stable for greater than six months from date of receipt at -20 to -70.

l          Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.

l          Avoid repeated freeze-thaw cycles.

 

   

    FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.  

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Last modified: 05/19/16