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Recombinant Human Flt3 Ligand  (rHuFlt3-L)

 

 

Description 

 

Flt3-ligand (flk2-ligand) stimulates the proliferation and colony formation of certain BM precursor cells including CD34+ cells. Recombinant Human Flt3-Ligand is a soluble 17.6 kDa polypeptide containing 155 amino acid residues, which comprises the extracellular domain of the transmembrane flt3-ligand protein.

 

 

 

Specifications and Use

 

Source

 

l          Escherichia coli.

 

Molecular Mass

 

l          17.6kDa

Purity

 

l          ³ 98% as determined by SDS-PAGE and HPLC analyses.

 

Biological Activity

l          rHuFlt3-L is fully biologically active when compared to standards. Its specific activity is ³1×106 units/mg.

 

Endotoxin Level

 

l          Less than 10EU/mg of rHuFlt3-L as determined by LAL method.

 

Formulation

 

l          Sterile filtered lyophilized (freeze-dried) powder, with 20mM Tris.HCl, 20mM NaClpH7.4

 

Solubility

 

 

l          It is recommended to reconstitute the lyophilized rHuFlt3-L in sterile distilled H2O to prepare a stock solution of no less than 100 mg/ml of the cytokine.  

 

Stability

l          The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20..

l          Reconstituted rHuFlt3-L should be stored in working aliquots at -20.

l          Avoid repeated freeze-thaw cycles.

 

Usage

l          FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.

 

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Last modified: 05/19/16