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PRV gE Antibody Distinguishing Test Kit

Brief
Pseudorabies Virus gE Antibody ELISA Test kit is used for the detection the Pseudorabies Virus gE antibody in the serum; the test kit is employed to detect anti PRV-gE antibody in the serum ,distinguish naturally infected from vaccined swine.
The test kit is made from the antigen coated microplate, enzyme conjugate (HRP goat-anti-pig IgG), and other reagents; it applies the indirect  ELISA  principle to test the antibody against PRV-gE antibody in the serum.

Principle
The test kit is the antigen coated microplate made through the protein expressed by Pseudorabies Virus gE gene. In the test, the diluted contrast serum and sample (to be tested) are added, and incubate; after that, if the sample exists PRV-gE specific antibody, it will be bound with the antigens of the microplate; eliminate the unbound antibody and other compositions by washing , and adding enzyme conjugate (anti-pig IgG-HRP) to specifically bind with the compound of antibody and antigen of the microplate. Then the unbound conjugate is removed by washing, add the TMB substrate in the well, to react with HRP into a blue product. After discontinuing the reaction by adding HF solution, test the OD value in each reaction well with  ELIASA  at 630nm wavelength.
 

Specifications
 

Reagents of the Test Kit

1 Antigen coated microplate........................................................................... 2 pieces (96 wells)

2 Negative control serum(green lid)............................................................... 1 tube (1ml/tube)

   Positive control serum(red lid)..................................................................... 1 tube (1ml/tube)

3 Anti-pig IgG-HRP combination(yellow lid).................................................1 bottle (22ml/bottle)

4 20× concentrated washing solution (white lid).......................................... 1 bottle (30ml/bottle)

5 Substrate A (red lid)/B (black lid)................................................................. 1 bottle each (12ml/bottle)

6 Stop solution (blue lid)................................................................................... 1 bottle (12ml/bottle)

7 Sample diluent solution (transparent lid)..................................................... 1 bottle (50ml/bottle)

 

Storage: store 2-8, not frozen.

Expiry date: 6 months

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Last modified: 05/19/16