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PRRSV RT-PCR test kit

Brief

This test was assessed for sensitivity and application as a rapid diagnostic tool by comparison with virus isolation and detection of PRRS virus antibody in blood. The RT-PCR test was slightly more sensitive than virus isolation for detection of virus in serum and markedly more sensitive than virus isolation from plasma from experimentally infected pigs.  

 Principle
RNA in refrigerated serum sample is extracted by TRIzol , The cDNA synthesis was performed with RNA template and specific primer . To produce fragment of PRRSV , degenerate at high temperature, anneal at low temperature, transcript at mid-temperature, cycle with Taq DNA , amplify million fold through 35 times cycle. Electrophoresize RT-PCR products , dyed by ETBR, generate specific fragments in the ultraviolet lights which can be seen by eyes.

Specifications
Components:

A         solution: Positive contro                       l 60 ul

B         solution: TriZol solution                       7ml

C         solution: CHCl3                                       3.5ml

D        solution: C3H3O                                       4ml

E        solution: CH3CH2OH                              12ml

F        solution: DEPC-Deionized water:         500ul

G        solution:RT-PCR buffer                         22.5ul X11

H       solution:sample buffer:                           50ul

 

Storage: B, C,D,H, stored at 4-8 ; A,E,F,G, stored at -20 ;don’t froze repeatedly.

Expiry date:3 months.

Specification:10 test

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Last modified: 05/19/16