CERTIFICATE OF ANALYSIS
#100 Cholera Toxin
from Vibrio cholerae
Price: 245 Euro
Each vial of cholera toxin, when
reconstituted to 1.0 ml with sterile distilled water, contains 1.0 mg of
protein in: 0.05 M Tris, 0.2 M NaCl,
0.001 M Na2EDTA
at pH 7.5.
Solubility of cholera toxin, in water, decreases with time, following
Each vial contains 1 mg determined by
extinction coefficient of 1.14 at 280 nm and 1 cm for 1 mg/ml.1
The preparation migrates as a single major
band in disc gel electrophoresis (nondenaturing conditions). The R280/260
of each preparation is reported.
When examined in an ADP-ribosylation assay,2,3
the specific enzymatic activity of each lot is at least as high as that of a
standard cholera toxin preparation.
Lyophilized powder stoppered under vacuum. It
is recommended that this material be stored at 4°C prior to and following
reconstitution. DO NOT
Good laboratory technique should be employed in the safe handling of this
product. This requires observing the following practices:
- Wear appropriate laboratory attire
including a lab coat, gloves and safety glasses.
- Do not mouth pipette, inhale, ingest or
allow to come into contact with open wounds. Wash thoroughly any area of the
body which comes into contact with the product.
- Avoid accidental autoinoculation by
exercising extreme care when handling in conjunction with any injection
- This product is intended for research
purposes by qualified personnel only. It is not intended for use in humans
or as a diagnostic agent. List Biological Laboratories, Inc. is not liable
for any damages resulting from the misuse or handling of this product.
FOR RESEARCH PURPOSES ONLY.
NOT FOR HUMAN USE.
- Spangler, Brenda D. (1992)
56 (4), 622-647.
- Tsai, S.-C., Noda, M., Adamik, R., Moss, J.,
and Vaughan, M. (1987) Proc. Natl.
Acad. Sci. USA 84,
- Tsai, S.-C, Noda, M., Adamik, R., Chang,
P.P., Chen, H.-C., Moss, J., and Vaughan, M. (1988)
J. Biol. Chem.
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