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Toxoplasmosis IgG Antibody ELISA kit

Brief

Porcine toxoplasmosis IgG antibody ELISA test kit is used for the detection of the toxoplasmosis antibodies in the serum.

Principle
 

Controlling this disease is based on method of serological diagnosis, test kit is enzyme immunoassay for the detection toxoplasmosis antibodies in porcine serum. Toxoplasmosis antigens are coated on 96-well plate, add sample into wells and then antibodies bound specifically by antigens, the unbound antibodies and other components will be removed by washing. Next add enzyme conjugate to specifically bind with the compound of antibodies and antigens on the microplate. The unbound enzyme conjugate will be removed by washing. Add the TMB substrate in the well, react to a blue product. At last, end the reaction by adding stop solution.

 

Specifications
 

Reagents

1 Antigen coated microplate .............................................................2 pieces (96 wells)

2 Negative control serum (green lid)................................................ 2ml/tube

   Positive control serum (red lid)...................................................... 2ml/tube

3 Enzyme conjugate (yellow lid).........................................................1 bottle (22ml/bottle)

4 20×concentrated washing solution (white lid)............................. 1 bottle (30ml/bottle)

5 Substrate A (red lid)......................................................................... 1 bottle (12ml/bottle)

   Substate B (black lid)........................................................................ 1 bottle (12ml/bottle)

6 Stop solution (blue lid)..................................................................... 1 bottle (12ml/bottle)

7 Sample diluent solution (transparent lid)....................................... 1 bottle (50ml/bottle)

 

Storage: store 2-8, not frozen.

Expiry date: 6 months

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Last modified: 05/19/16