GENTAUR Contact Gentaur

Up

GENTAUR Survey

GENTAUR EUROPE

+32 1658 9045
+32 1650 9045

BELGIUM1

tel +32 2 732 5688
fax +32 2 732 4414
info@gentaur.com
Av. de l' Armée 68
B-1040 Brussels

France

tel 01 43 25 01 50
fax 01 43 25 01 60
9, rue Lagrange
75005 Paris

Italy

tel 02 36 00 65 93
fax 02 36 00 65 94
20135 Milano

Germany

tel +32 16 58 90 45
fax +32 16 50 90 45
Forckenbeckstraße 6
D-52074 Aachen

Japan

tel +81 78 386 0860
fax +81 78 306 0296
Minaatojimaminami-manchi
Chuo-ku, Kobe
065-0047


PPV antibody ELISA Test Kit

 

Brief

The porcine parvovirus (PPV) ELISA test kit is used for the detection the porcine parvovirus antibodies in swine serum; assessment the immunity conditions against porcine parvovirus in the pig farm; investigation epidemiology of the porcine parvovirus.

The test kit is made from the antigen coated microplate, HRP goat-anti-pig IgG, and other reagents; it applies the indirect  ELISA  principle to test the antibodies against porcine parvovirus in pig serum.

 

Principle

The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the porcine parvovirus. In the test, the diluted contrast serum and sample are added, then incubate. If porcine parvovirus specific antibodies exist in the sample , it will be bound with the antigens on the microplate, Remove the unbound antibodies and other components by washing. Add IgG-HRP to specifically bind with the compound of antibody and antigen on the microplate. Then the unbound conjugates will be removed by washing. Then add the TMB substrate to the well, react with HRP to a blue product. Stop the reaction by adding HF solution, measure the OD630nm value.

 

Specifications

Reagents of the Test Kit

1 Antigen coated microplate……………………………………….2 pieces (96 wells)

2 Negative control serum (green lid)…………………….……...…1 tube (1ml/tube)

Positive control serum (yellow lid)………………………...……....1 tube (1ml/tube)

3 Anti-pig IgG-HRP combinationyellow lid…………….…...1 bottle (22ml/bottle)

4 20× concentrated washing liquid (white lid)……………………1 bottle (30ml/bottle)

5 Substrate A (red lid) …………………………….…………..….....1 bottle each (12ml/bottle)

Substrate B (black lid)……………………………………..…..….....1 bottle each (12ml/bottle)

6 Stop solution (blue lid) ………………………………….…….…..1 bottle (12ml/bottle)

7 Sample diluent (transparent lid)………………………...…………1 bottle (50ml/bottle)

 

Storage: store 2-8, not frozen.

Expiry date: 6 months

Home Up

send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Site powered by Acid Dragon (AC)
Last modified: 05/19/16