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Nitrofuran (SEM) ELISA Kit

Brief
SEM ELISA kit, Semicarbazide (SEM) ELISA Test Kit

Principle
This test kit is based on the competitive enzyme immunoassay for the detection of semicarbazide (SEM) in the tissue(chicken, fish, shrimp), milk, honey, whole egg. The coupling antigen is pre-coated on the micro-well stripes. The SEM in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against SEM. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample is inversely proportional to the SEM in the sample. This value is compared to the standard curve and the content of the corresponding SEM is subsequently obtained.

Specifications
Sensitivity : 0.1ppb
Detection limit:
Tissue ,honey ,egg 0.2 ppb
  Fish, shrimp(some interference in shrimp and fish) 0.3 ppb
Recovery rate
  Shrimp and fish 85±10%
Honey ,chicken meat /liver,whole egg 75±15%
Cross-reaction rate:
  SEM 100%
AHD ﹤0.1%
AMOZ ﹤0.1%
AOZ ﹤0.1%

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Last modified: 05/19/16