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JEV ELISA test kit

Brief

The test kit is made from the antigen coated microplate, enzyme conjugations(HRP goat-anti-pig IgG), and other reagents. It applies the indirect  ELISA  principle to test the antibodies against porcine encephalitis virus in pig serum.

 

Principle
The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the porcine encephalitis virus. In the test, the diluted control serum and sample are added, then incubate. If porcine encephalitis Virus specific antibodies exists in the sample, It will be bound with the antigens on the microplate, but the unbound antibodies and other components will be removed by washing. Next, add enzyme conjugations(anti-pig IgG-HRP) to specifically bind with the compound on the microplate. Then the unbound conjugations will be removed by washing. Add the TMB substrate in the well, react with HRP to a blue product. At last, end the reaction by adding stop solution.

Specifications

Reagents of the Test Kit

1 Antigen coated microplate.................................................................... 2 pieces (96 wells)

2 Negative control serum(green lid) ....................................................... 1 tube (1ml/tube)

Positive control serum (red lid)................................................................ 1 tube (1ml/tube)

3 Anti-pig IgG-HRP combination(yellow lid)......................................... 1 bottle (22ml/bottle)

4 20×concentrated washing liquid (white lid)........................................ 1 bottle (30ml/bottle)

5 Substrate A (red lid) .............................................................................. 1 bottle (12ml/bottle)

Sustrate B (black lid)................................................................................. 1 bottle (12ml/bottle)

6 Stop solution (blue lid).......................................................................... 1 bottle (12ml/bottle)

7 Sample diluent solution(transparent lid)............................................. 1 bottle (50ml/bottle)

 

 

Storage:       store 2-8, not frozen.

Expiry date: 6 months

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Last modified: 05/19/16