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HCV Antibody ELISA Test Kit

 Brief
The Hog Cholera Virus ELISA Antibody Test Kit is used for the detection the Cholera Virus antibody in swine serum; assessing the immunity conditions against Cholera Virus in the pig farm; and for the investigation of the epidemiology of the Cholera.
The test kit is made from the antigen coated microplate, HRP goat-anti-pig IgG, and other reagents; it applies the indirect  ELISA  principle to test the antibody against Cholera virus in pig serum.

Principle
The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the Hog Cholera Virus. In the test, the diluted contrast serum and sample (to be tested) are added, and incubate; after that, if the sample exists Cholera virus specific antibody, it will be bound with the antigens of the microplate; eliminate the unbound antibody and other compositions by washing , and adding IgG-HRP to specifically bind with the compound of antibody and antigen of the microplate. Then the unbound conjugate is removed by washing, add the TMB substrate in the well, to react with HRP into a blue product. After discontinuing the reaction by adding HF solution, test the OD value in each reaction well with ELIASA at 630nm wavelength. Specifications
 

Reagents of the Test Kit

1 Antigen coated microplate......................................................................... 2 pieces (96 wells)

2 Negative control serum (green lid) ........................................................... 1 tube (1ml/tube)

   Positive control serum serum (red lid)....................................................... 1 tube (1ml/tube)

3 Anti-pig IgG-HRP combination(yellow lid)............................................... 1 bottle (22ml/bottle)

4 20×concentrated washing solution (white lid).......................................... 1 bottle (30ml/bottle)

5 Substrate A (red lid) ..................................................................................... 1 bottle (12ml/bottle)

  Substrate B (black lid).................................................................................... 1 bottle (12ml/bottle)

6 Stop solution(blue lid)................................................................................... 1 bottle (12ml/bottle)

7 Sample diluent solution(transparent lid)..................................................... 1 bottle (50ml/bottle)

 

Storage: store 2-8, not frozen

Expiry date: 6 months

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Last modified: 05/19/16