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Broth™ is a proprietary medium formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli.  This medium is derived from the Superior Broth™ formula but made without animal-derived materials.  The medium is a complex, rich formulation which supplies a nitrogen source, vitamins, and moderate glucose levels.  The medium is buffered at pH 7.2±0.2 to prevent acidification of the medium during metabolism of the glucose.  For expression of recombinant proteins, induction should be done at cell densities twice that of LB Broth.  Cell yields are typically about 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain.  Plasmid copy numbers are the highest among the AthenaES™ family of media.  This medium is an excellent choice for large-scale plasmid preparations.  Biomass yields will depend on the process employed and the strain.

Formula – Proprietary

Method of Preparation

  1. Dissolve 35 grams in 1 liter deionized water.

  2. Dispense as required.

  3. Autoclave at 121°C for 15 min.

Media

Unit Size

Cat. No.

Superior Prime Broth™

500 g

2 pack

6 packa

0111-1

0111-2

0111-6

 a Six pack sold as six 500 g containers to ensure freshness during storage, prevent unintentional contamination during use and prevent caking of powder.

 

Power Prime Broth™

Cat. No. 0112

Power Prime Broth™ is a proprietary medium formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli.  This medium is certified "animal product free" and is a derivative of the Power Broth™ formula.  The medium is a complex, rich formulation which supplies amino acids, vitamins, and a carbon source at higher levels than Terrific Broth5.  The medium is buffered at pH 7.2±0.2.  For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth.  Cell yields are typically 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain.  The amount of soluble protein accumulated for otherwise insoluble proteins is increased by growing in Power Broth™.  This medium is suitable as the base for fermentation cultures.  Biomass yields will depend on the process and the strain employed.

Formula – Proprietary

Method of Preparation

  1. Dissolve 52 grams in 1 liter deionized water.

  2. Add 4 ml of glycerol.

  3. Dispense as required.

  4. Autoclave at 121°C for 15 min

Media

Unit Size

Cat. No.

Power Prime Broth™

500 g

2 pack

6 packa

0112-1

0112-2

0112-6

 a Six pack sold as six 500 g containers to ensure freshness during storage, prevent unintentional contamination during use, and prevent caking of powder.

 

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Last modified: 05/19/16