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Diazepam ELISA Test Kit

Brief
Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Diazepam in the feed,urine, liver, meat. The coupling antigen is pre-coated on the micro-well stripes. The Diazepam in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Diazepam antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Diazepam in it. This value is compared to the standard curve and the Diazepam concentration is subsequently obtained.

Specifications

Specification: 96wells /kit

Sensitivity: 0.1 ppb

Detection limit

Tissue 1 ppb

Urine  1 ppb

Feed  10 ppb

Recovery rate

Urine  80 ±10%

Feed  75 ±10%

Tissue(meat, liver)   85 ±10%

Cross-reactions rate

Diazepam  100%

Nitrazepam 7.6%

Oxazepam  8.8%

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Last modified: 05/19/16