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Diazepam ELISA Kit

Brief
Diazepam ELISA Test Kit

Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Diazepam in the feed,urine, liver, meat. The coupling antigen is pre-coated on the micro-well stripes. The Diazepam in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes, to interact with the antibodies against Diazepam. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Diazepam in the sample. This value is compared to the standard curve and the content of the corresponding Diazepam is subsequently obtained.

 Specifications
Sensitivity : 0.1ppb
Detection limit
  Animal tissue 1ppb
  Urine 1ppb
  Compound feed 10ppb
  Concentrated feed 20ppb
  Premixed feed 20ppb
Recovery rate
  Urine 80 ±10%
  Feed 75 ±10%
  Meat,liver(pork) 75 ±10%
Cross-reactions rate
  DiaZepatn 100%
  Nitrazepam 7.6%
  Oxazepam 8.8%

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Last modified: 05/19/16