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 A

NTI-CONJUGATED GLYCINE

DESCRIPTION

Polyclonal antisera were raised in rabbits after immunization with the conjugates:

Glycine-glutaraldehyde-carriers.

CONTENTS

The antibody is supplied in a unit size of 50

ml in PBS with 50% glycerol.

STORAGE AND HANDLING

When this product is stored undiluted at -20

°C or lower, it is stable for at least 2 years. Multiple freezing

and thawing is only possible up to 5 times. The antibodies can be prediluted 10x in PBS containing 0.1%

merthiolate or a mixture of PBS/glycerol (1:1, vol./vol.). At 4

°C these solutions are stable for 2 months.

SPECIFICITY

Using a conjugate glycine-glutaraldehyde-protein, antibody specificity was determined in an ELISA test by

competition experiments with the following compounds:

Compound Cross-reactivity ratio

(a)

Glycine-G-TH 1

b

-Alanine-G-TH 1 : 107

GABA-G-TH 1 : 501

Taurine-G-TH 1 : 1,000

Aspartate-G-TH 1 : 20,000

Glutamate-G-TH 1 : > 20,000

Glycine=G=TH (b) 1 : > 100,000

Glycine 1 : > 100,000

(a) : Glycine-G-PL concentration/unconjugated or conjugated aminoacid concentration at half displacement ;

(b) : Non-reduced conjugate ;

G = Glutaraldehyde, TH = Thyroglobulin.

RECOMMENDED DILUTION

The anti-conjugated glycine antibodies can be diluted 1:2,000 to 1:5,000.

REFERENCES

• CAMPISTRON G., BUIJS R.M. and GEFFARD M., Glycine neurons in the brain and spinal cord. Antibody production and immunocytochemical

localization. Brain Res., (1986) 376, 400-405.

• PEYRET D., CAMPISTRON G., GEFFARD M. and ARAN J.M., Glycine immunoreactivity in the brain-stem auditory and vestibular nuclei of the

guinea pig. Acta Otolaryngol. (Stockh), (1987) 104, 71-76.

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Last modified: 05/19/16