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Product Name 

MAP2 antibody - Neuronal Marker

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MAP2 - Neuronal Marker

Immunogen 

Cow brain MAP 2 purified from the material that pellets with GTP polymerized tubulin (see references).

Cross Reactivity 

Cross-reacts with Cow, Human, Mouse and Rat.
Not yet tested in other species.

Tested applications 

Western blot, Immunofluorescence

Application notes 

IF: Use at a dilution of 1/30000.
WB: Use at a dilution of 1/300000. Detects a band of approximately 280 kDa, corresponding to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

   

Cellular localisation 

Cytoplasmic

Research areas 

Neuroscience, Cytoskeleton

Relevance 

Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one alpha subunit and one beta tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins, which includes the protein t and MAP 1, MAP 2, MAP 3, MAP 4 and MAP 5. MAP 2 is found only in neuronal cells and specifically in the perikarya and dendrites. Antibodies to MAP2 are therefore excellent markers of neuronal cells and neuronal dendrites.

 

Alternative names for MAP2 antibody - Neuronal Marker (ab5392)

Database links 

 

GeneCard

    

GC02P209169  (Human)
 

Locus Link

    

4133  (Human)
17756  (Mouse)
25595  (Rat)
 

Omim

    

157130  (Human)
 

SwissProt

    

P11137  (Human)
P20357  (Mouse)
P15146  (Rat)
 

Unigene

    

167  (Human)
256966  (Mouse)
10484  (Rat)
 

 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Storage buffer 

Preservative: 5mM Sodium Azide
Constituents: PBS, Whole Serum

Purification notes 

Lipid extraction from egg yolk, followed by ammonium sulphate precipitation.

Form 

Liquid,                                 200 µl                                 325 Euro

Concentration 

19.00 mg/ml

Storage instructions 

Aliquot and store at -20°C or -80°C.
Avoid repeated freeze / thaw cycles.

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Last modified: 05/19/16