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TACSTM Annexin V Kits

Annexin V protocol
Annexin V brochure

TACS Annexin V Kits allow rapid, specific, and quantitative identification of apoptosis in individual cells. Annexin V is a calcium-dependent phospholipid binding protein. During apoptosis, an early and ubiquitous event is the exposure of phosphatidylserine at the cell surface. Trevigen offers annexin V conjugated to either FITC or to biotin for the detection of cell surface changes during apoptosis. The annexin V conjugates are supplied with an optimized Binding Buffer and propidium iodide. Propidium iodide may be used on unfixed samples to determine the population of cells that have lost membrane integrity, an indication of late apoptosis or necrosis.

Features:   
  • Fast, sensitive detection of apoptotic cells.
  • Includes propidium iodide for discriminating apoptotic and necrotic (or late apoptotic) cells.
  • Annexin V-Biotin provides flexibility in fluorophore choice.
  • Applications:   
  • Flow cytometry
  • In situ detection of apoptotic cells
  •   Annexin V FITC Kits
    Components:    Component Size Price
      Annexin V FITC (A) 100 µl 323 €
      10X Binding Buffer (A) 8 ml 84 €
      Propidium Iodide (A) 1 ml 84 €
      Annexin V FITC (B) 250 µl 665 €
      10X Binding Buffer (B) 20 ml 103 €
      Propidium Iodide (B) 2.5 ml 103 €
     
      (A) 4830-01-K Annexin V FITC Kit 100 Samples         347 €
    (B) 4830-250-K Annexin V FITC Kit 250 Samples       690 €
     
      Annexin V Biotin Kits
    Components:    Component Size Price
      10X Binding Buffer (A) 8 ml 84 €
      Propidium Iodide (A) 1 ml 84 €
      10X Binding Buffer (B) 20 ml 103 €
      Propidium Iodide (B) 2.5 ml 103 €
      Annexin V Biotin (A) 100 µl 371 €
      Annexin V Biotin (B) 250 µl 694 €
     
      (A) 4835-01-K Annexin V Biotin Kit 100 Samples       381 €
    (B) 4835-250-K Annexin V Biotin Kit 250 Samples     708 €
     
    Storage:    Stable for at least one year when stored at 4°C.
     
    References:    1. Fadock, V.A., D.R. Voelker, P.A .Campbell, J.J. Cohen, D.L. Bratton, and P.M. Henson. 1992. Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J Immunol 148:2207-2216.
    2. Tait, J.F., and D. Gibson. 1992. Phospholipid binding of Annexin V: Effects of calcium and membrane phosphatidylserine content. Arch Biochemiand Biophys 298(1):187-191.
    3. Meers, P., and T. Mealy. 1993. Calcium-dependent Annexin V binding to phospholipids: stoichiometry, specificity, and the role of negative charge. Biochemistry 32:11711-11721.

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