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Human & Mouse/ Rat Adiponectin ELISA Kits


K1002-1-BR  Human & Mouse/ Rat Adiponectin ELISA Kits   549 Euro


Proven system for sensitive, non-radioactive detection of adiponectin
 

  • Simple, non-radioactive procedure
     
  • Highly selective adiponectin-specific detection
     
  • Sensitive linear signal over a broad linear range
     

As the first non-radioactive detection kits for adiponectin, B-Bridge's Human and Mouse/Rat Adiponectin ELISA Kits (Cat. # K1001-1 and K1002-1) enable sensitive and convenient detection of adiponectin from serum samples, cell extracts, or conditioned medium. Over the past two years, B-Bridge's Kits have proven their effectiveness at providing quality results in experiment after experiment. We have the references to prove it!


Sensitive detection with an easy, non-radioactive procedure

Starting at concentrations as low as 0.25 ng/ml, B-Bridge's Adiponectin ELISA Kits provide you with a reproducible and accurate linear response over a broad concentration range. As shown, each dilution results in a linear and well-correlated decrease in absorbance. Our high-affinity, highly specific primary antibody and robust biotin/streptavidin-HRP detection procedure provide assay sensitivity and range that is very comparable to that obtained with typical radioimmunoassay assays (RIA).

 

 
Linear dilution of murine adiponectin detected using the Mouse/Rat Adiponectin ELISA Kit.   High correlation of B-Bridge's ELISA Adiponectin Kit with standard Human Adiponectin RIA Kit
(click on figure to see more correlation data)

 

 

 

 

 

Obtain accurate results - specific for adiponectin

The primary antibody used in B-Bridge's Adiponectin ELISA Kits is a highly selective, high-affinity antibody that specifically targets either human adiponectin, or mouse and rat adiponectin (depending on which Kit you are using). As the data below show, our systems are very selective for the target adiponectin proteins - even when challenged with adiponectin from highly similar mammalian species.

 
Selectivity of the Human ELISA Kit
Adiponectin signal levels of non-human sera (diluted 1,111-fold) are virtually at background levels.
 

 

 
Selectivity of the Murine/Rat ELISA Kit
 

Adiponectin signal levels of sera from other animals (diluted 1,111-fold) are similar to background levels.
 

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